Journal: iScience

Article Title: The STING1-MYD88 complex drives ACOD1/IRG1 expression and function in lethal innate immunity

doi: 10.1016/j.isci.2022.104561

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Article Snippet: Concentrations of proteins in the supernatant were determined by a BCA Protein Assay Kit (7780, Cell Signaling Technology).

Techniques: Recombinant, Control, Lysis, Enzyme-linked Immunosorbent Assay, cDNA Synthesis, Bicinchoninic Acid Protein Assay, Magnetic Beads, Chromatin Immunoprecipitation, Purification, CCK-8 Assay, shRNA, Software, Western Blot

Journal: Frontiers in Immunology

Article Title: GPR182 is a broadly scavenging atypical chemokine receptor influencing T-independent immunity

doi: 10.3389/fimmu.2023.1242531

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Article Snippet: Anti-mCXCL13 , 143614 , 1:1000 , RnD Systems , MAB470.

Techniques: Concentration Assay

Journal: Cell Communication and Signaling : CCS

Article Title: CD69 + CD103 + CD8 + tissue-resident memory T cells possess stronger anti-tumor activity and predict better prognosis in colorectal cancer

doi: 10.1186/s12964-024-01990-3

Figure Lengend Snippet: Relationship of different CD8 + TRM subset with B cells and TLS in CRC tumor tissues. ( A ) Volcano plot showing differentially expressed genes between DP CD8 + TRM subsets and SP CD8 + TRM subsets of the GSE108989 dataset. ( B ) The FFPE CRC tumor tissue slides from patients with CRC were stained with DAPI (blue), CD20 (green), CD8 (yellow), CD103 (orange), and CD69 (red). ( C ) Relationship between the proportion of different CD8 + TRM cells and the proportion of B cells in CRC tumor tissues detected by flow cytometry ( n = 40) and relationship between the proportion of different CD8 + TRM cells and the count of B cells and the count and area of TLS in CRC tumor tissues detected by mIHC ( n = 64). Statistical analysis was performed by Pearson correlation analysis. ( D ) Schematic overview of the transwell migration assay design. ( E ) B cell migration rate when cultured either alone or with different CD8 + TRM subsets ( n = 10) and B cell migration rate when cultured with DP CD8 + TRM cells with or without anti-CXCL13 neutralizing antibody ( n = 7). Statistical analysis was performed by non-parametric Wilcoxon’s matched-pairs tests and Friedman test with Dunn’s multiple comparisons test. (* p < 0.05; ** p < 0.01)

Article Snippet: To disrupt CXCL13/CXCR5 interactions during chemotaxis, neutralizing antibody against CXCL13 (2 µg/mL, MAB801, R&D Systems) was added to the culture medium.

Techniques: Staining, Flow Cytometry, Transwell Migration Assay, Migration, Cell Culture

Journal: Advanced Science

Article Title: Tertiary Lymphoid Structure in Dental Pulp: The Role in Combating Bacterial Infections

doi: 10.1002/advs.202406684

Figure Lengend Snippet: Multiplex immunofluorescence staining of TLS in human dental pulp. a) Distribution of CD20 + cells, CD34 + cells, CCL3 + cells, and CXCL13 + cells in healthy dental pulp (Healthy 1 and 2). Colors were indicated as follows: Red (CD34), Yellow (CD20), Magenta (CCL3), Green (CXCL13), and Blue (DAPI). Od, odontoblast. Scale bars = 50 µm. b) Distribution of TLS marked by CD20 + cells, CD34 + cells, CCL3 + cells, and CXCL13 + cells in inflamed dental pulp (Pulpitis 1, 2, 3, and 4). Color coding as above. The area indicated by the green arrow represented the CCL3 expression. Od, odontoblast. Scale bars = 50 µm.

Article Snippet: Anti‐CXCL13 (NBP3‐07988) antibody was purchased from Novus Biologicals (Colorado, CA, USA).

Techniques: Multiplex Assay, Immunofluorescence, Staining, Expressing